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VitaVitro 1-step Culture Medium
VitaVitro 1-step Culture Medium is designed for continuous culture from Day 1 to 5, with no dish or medium change required.
ADVANTAGES

VitaVitro 1-step Culture Medium combined with VitaVitro WOW Dishes maximizes

opportunity to produce high-quality embryos in vitro.

WELL OF THE WELL (WOW) CULTURE DISHES
VitaVitro WOW (Well-of-Well) Culture Dish is designed for culturing embryos following fertilization to the blastocyst stage.
 
TO CREATE A CULTURE ENVIRONMENT
 
The VitaVitro WOW Culture Dish is an advanced culture dish designed for IVF that allows group culture of embryos while maintaining individual separation between the embryos and re-creates the three-dimensional in vitro environment for embryos. The semi-globe connected to straight walls – a kind of inverted sugar-loaf shape microwells allow dilution of light waste materials and increased concentration of heavy autocrine and paracrine factors around the embryos. The flat-bottom microwells are designed for individual microscopic evaluation or Time-Lapse follow-up, providing options for special needs.

CLINICAL DATA

In 2021, a prospective, self paired study was carried out in Europe, including 24 subjects aged 30-39 years, with a total of 206 oocytes.

The results shows that in the test group, the fertilization rate was 81%, the blastocyst formation rate was 65%, and the high-quality blastocyst formation rate was 87%.

*blastocyst formation rate = (D5/6 Total number of blastocyst) / 2PN Number of fertilized oocytes *100%
*High quality blastocyst formation rate =Number of High-quality blastocysts / Number of formatted blastocysts*100%

VIDEO

Embryo Production System

This video demonstrated how Well-of-the-well culture dish works.

References

1. Vaita G., Peura T.T., Holm P., Páldi A., Greve T., Trounson A.O., Callesen H. New method of culture of zona-included or zona-free embryos: the well of the well (WOW) system. Mol. Reprod. Dev. 2000; 55(3): 256–264. DOI:10.1002/(sici)1098-2795(200003)55:3<256::aid-mrd3>3.0.co;2-7.

2. Vajta G., Kőrösi T., Du Y., Nakata K., leda S., Kuwayama M., Nagy Z.P. The Well-of-the-Well system: an efficient approach to improve embryo development. Reprod Biomed Online. 2008; 17(1): 73-81. DOI: 10.1016/S1472-6483(10)60296-9.

3. Vajta G., Nagy Z.P., Cobo A., Conceicao J., Yovich J. Vitrification in assisted reproduction;myths,mistakes, disbeliefs and confusion. Reprod Biomed Online. 2009; 19 Suppl 3: 1-7. DOI: 10.1016/S1472-6483(10)60278-7.

 Ordering information
Product NameVolume(mL)Catalog No.
VitaVitro 1-step Culture Medium (With HSA)
10
30
V004010
V004030
VitaVitro Well-of-the-well (WOW) Culture Dish
___
___
 Material safety data sheet
VitaVitro 1-step Culture Medium MSDS
 Quality control
VitaVitro 1-step Culture Medium (With HSA)

Osmotic pressure：250-290 mOsm/kg

pH: 7.2-7.6

Endotoxin：< 0.25EU/mL

Sterility test：Passed

Mouse embryo assay (1-cell)：≥ 80% development to blastocyst at 96 hours