- VitaVitro Vitrification Kit & VitaVitro Warming Kit
- Open Pulled Straw
If the solution freezes, we recommend that you discard it immediately. As stated in the User Guide, the product should be stored at a temperature of between 2–8°C. When stored unopened within this range, the product will remain stable and ready to use until the date shown on the bottle. Media should not be exposed to temperatures outside this range at any time.
Yes. VitaVitro vitrification and warming solutions can be used to vitrify or warm embryos that have been cultured in any CE- or FDA-approved embryo culture media.
Once opened, it is recommended to use the solution within one day, assuming it is used under aseptic conditions in closed vials. Discard unused residual solution.
If used in accordance with the User Guide, one kit can be used for 5 vitrification cycles and 1–2 warming cycles.
Under normal circumstances:
No. VitaVitro vitrification and warming media do not contain antibiotics due to the increased risk of intracellular accumulation and toxic damage during the cryopreservation process. The process of equilibration and dilution is relatively short, and all media used before and after cryopreservation should contain antibiotics. As stated in the User Guide, vitrification and warming media, like all other aspects of the procedure, should be handled using aseptic technique. Based on extensive experience worldwide, these measures are sufficient to prevent infections.
We recommend approximately 1 µl of vitrification solution for sample loading. Using an insufficient amount of solution may affect the capillary suction and the formation of a solution column at the end of the straw, while using too much solution may compromise the cooling and warming rate.
A maximum of 3 samples in one straw is recommended.
The recommended solution is to insert a 20 μl micropipette with a narrow plastic tip into the wide end of the OPS and gently expel air into the OPS to eject the solution column into the warming dish. To avoid delays, we suggest having a pipette and tip ready for use before starting the procedure.
Sealing must be performed with a handheld heat sealer adjusted to 100–120°C. Note: Approximately 70% of the container straw should be kept immersed in LN2 during the sealing process. VitaVitro offers a purpose-designed handheld sealer for this procedure.