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CRYOPRESERVATION

VitaVitro Biotech’s uniquely effective vitrification and warming solution adopts Professor Vajta’s pioneering open-pulled straw (OPS) method. Highly efficient for human MII-phase oocytes and embryos of different stages of development including early stage, morulae, and blastocysts.
To date, OPS has resulted in:

  • The first human baby after oocyte vitrification (2001)
  • The first cloned animal after embryo cryopreservation (2003)
  • The first calf after cryopreservation of immature oocytes (2004)
  • The highest survival-developmental rates after cryopreservation of human ES cells (2008)
  • Vitrification Solutions
  • Warming Solutions

The Vitrification Kit is a unique approach to the vitrification of human blastocysts for assisted reproduction technologies (ART).

CONTENTS
CODE PRODUCT NAME CONTENTS QUALITY CONTROL
LD-1.0 VitaVitro Vitrification Kit HHM (human holding media) 1.8ml×1
HW1 (human Vitrification Solution 1) 1.5ml×2
HW2 (human Vitrification Solution 2) 1.0ml×1
pH 7.2-7.6
Endotoxin < 0.25EU/ml
Mouse Embryo Assay ≥80%
Osmolarity
Sterility Test

The Vitrification Warming Kit is used for warming vitrified human blastocysts for assisted reproduction technology (ART).

CONTENTS
CODE PRODUCT NAME CONTENTS QUALITY CONTROL
JD-1.0 VitaVitro Warming Kit HHM (human holding media) 1.8ml×1
HW1 (human warming media) 1.5ml×2
HW2(human warming media) 1.0ml×1
pH 7.2-7.6
Endotoxin < 0.25EU/ml
Mouse Embryo Assay ≥80%
Osmolarity
Sterility Test

The following chart shows the high embryo survival rate achieved by VitaVitro’s vitrification and warming solutions:

Center of Assisted Reproduction | Bedford, Texas, United States | Dr. Martin Langley

DATA
FROZEN EMBRYO SURVIVAL
FROZEN EMBRYO TRANSFER CLINCAL OUTCOMES
SURVIVAL,BLASTOCYST AND HATCHING RATES OF MOUSE EMBRYOS PRODUCED IN VITRO FROM VITRIFIED-WARMED OOCYTES
Reference
  1. Open versus closed systems for vitrification of human oocytes and embryos.
    Vajta G, Rienzi L, Ubaidi FM. I Reprod Biomed Online. 2015 Apr;30(4):325-33.
  2. Vitrification in human and domestic animal embryology;work in progress..
    vajta G. I Reprod Fertil Dev. 2013;25(5);719-27.
  3. Vitrification in assisted reproduction;myths,mistakes, disbeliefs and confusion..
    Vajta G, Nagy ZP, Cobo A, Conceicao J, Yovich J. I Reprod Biomed Online.2009;19 Suppl 3:1-7.
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